Journal: Biomolecules

Article Title: Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes

doi: 10.3390/biom12071008

Figure Lengend Snippet: Mouse adipose stem cell (ASC) isolation and confirmation. ( A ). Morphology of ASC shows regular fibroblastic cell morphology. ( B ). Flow cytometric analysis of cultured ASC at 3rd passage. ASC was positive for CD29, CD90, and CD105 and negative for CD45, which confirms the purity of ASC. ( C ). Immunofluorescence staining confirmed the expression of CD29 and CD105 in ASCs. The nucleus was stained with DAPI. ( D , E ). Confirmation of the multipotency of ASC. ( D ). Adipogenic differentiation. Oblate-like cell morphology and numerous lipid droplets can be visualized without specific staining. E. Osteogenic differentiation. The cells developed a cluster-shaped structure and showed positive red staining with alizarin red staining, confirming the multipotency of ASC.

Article Snippet: The cells were then incubated with Alexa fluor 647 anti-mouse CD29 (Biolegend, Tokyo, Japan), Alexa fluor 488 anti-mouse CD105 (Biolegend), phycoerythrin anti-mouse CD90 (Novus Biologicals, Littleton, CO, USA), FITC anti-mouse CD45 (Invitrogen, Waltham, MA, USA) fluorescent conjugated antibodies or isotype control Alexa flour 647 anti-mouse IgG1(Biolegend), and FITC anti-mouse IgG1 (Biolegend) antibodies according to recommended concentration and incubated for 1 h at RT.

Techniques: Isolation, Cell Culture, Immunofluorescence, Staining, Expressing